Extended Data Fig. 5: Conformational changes between EMC-bound and CaVα2δ-bound CaV1.2(ΔC)/CaVβ3.
From: EMC chaperone–CaV structure reveals an ion channel assembly intermediate
Superposition of CaV1.2 from the EMC:CaV1.2(ΔC)/CaVβ3 and CaV1.2(ΔC)/CaVβ3/CaVα2δ-1 complexes showing a, VSD conformational changes. b, PD conformational changes showing the superposition from ‘a’. Insets show each PD. Elements CaV1.2 from the EMC complex are: VSD I/PD I (yellow orange), VSD II/PD II (firebrick), VSD III/PD III (lime), VSD IV/PD IV (marine). CaV1.2 (slate) and CaVβ3 (violet) from CaV1.2(ΔC)/CaVβ3/CaVα2δ-1 and CaVβ3 from the EMC (light teal) are semi-transparent. c, Superposition of CaVβ3 and the CaV1.2 AID helix from the EMC:CaV1.2(ΔC)/CaVβ3 and CaV1.2(ΔC)/CaVβ3/CaVα2δ-1 complexes. Location of EMC8 is indicated by the orange oval. Red arrows in ‘a-c’ indicate conformational changes between CaV1.2(ΔC)/CaVβ3/CaVα2δ-1 and EMC:CaV1.2(ΔC)/CaVβ3. d, Comparison of IIS0 and surrounding regions in the EMC complex (VSDII, firebrick; AID (yellow orange), and CaVβ3 (light teal)) their corresponding elements in CaV1.2(ΔC)/CaVβ3/CaVα2δ-1 (slate). e, Interactions between VSD II:PD III in the CaVβ:AID:VSD II:PD III subcomplex from the EMC:CaV1.2(ΔC)/CaVβ3 structure.
