Extended Data Fig. 8: Exemplar purification of CaV subunit combinations.
From: EMC chaperone–CaV structure reveals an ion channel assembly intermediate
a-c Superose 6 Increase 10/300 GL chromatogram and peak fraction SDS-PAGE for: a, CaV1.2(ΔC)/CaVβ3 and b, CaV1.2(ΔC). c, Relative detection by mass spectrometry from ‘a’ and ‘b’ of EMC proteins with respect to CaV1.2(ΔC) across 3 replicates. d, Superose 6 Increase 10/300 GL chromatogram and peak fraction SDS-PAGE for CaVβ3. CaVβ3-NK and CaVβ3-SH3 are CaVβ3 proteolytic fragments. e, Absolute detection of EMC proteins and CaVβ3 by mass spectrometry following expression and purification of CaVβ3. f, Superose 6 Increase 10/300 GL chromatogram and peak fraction SDS-PAGE for CaV1.2/CaVβ3. Magenta bars in ‘a’ ‘b’, ‘d’, and ‘f’ mark peak fraction. g, Absolute detection of CaV1.2, CaV1.2(ΔC), CaVβ3, and EMC proteins from ‘a’ and ‘f’. Error bars are calculated as SEM. ND denotes not detected.
