Extended Data Fig. 9: Functional and biochemical characterization of CaV mutants.
From: EMC chaperone–CaV structure reveals an ion channel assembly intermediate
a, Voltage dependent activation (left) and I-V relationships (right) for the indicated channels. Parentheses indicate ‘n’ independent cells examined over >2 independent experiments. b, Superose 6 Increase 10/300 GL chromatogram and peak fraction SDS-PAGE for CaV1.2(ΔC)/CaVβ3(11A). Magenta bar marks peak fraction. c, Relative detection by mass spectrometry from ‘b’ of EMC proteins with respect to CaV1.2(ΔC) across 3 replicates. Data for CaV1.2(ΔC)/CaVβ3 are from Extended Data Fig. 8c. ‘ND’ indicates ‘not detected. Data in ‘a’ and ‘c’ are presented as mean ± SEM.
