Fig. 1: Mast cells are essential for antigen-avoidance behaviour in type 2 immunized mice.
From: Mast cells link immune sensing to antigen-avoidance behaviour
a,b, Type 2 immunization scheme, experimental timeline for avoidance test (a) and IntelliCage setup with bottle positioning (b). Purple symbols indicate water, yellow symbols indicate egg white water with sucrose. c, Egg white water preferences of alum-immunized and OVA-alum-immunized BALB/c Cpa3+/+ and Cpa3Cre/+ mice displayed as percentage of egg white water intake over total water intake (colour scale indicates percentages) during the course of experiments 1 and 2. Each row corresponds to an individual mouse. d, Egg white water preference displayed as number of egg white water licks over total number of water licks versus time. Data are presented as mean ± s.e.m. e, Egg white water preference displayed as the fraction of egg white water intake over total water intake as an average per day for the duration of the experiment. f,g, Serum amounts of anti-OVA IgE (f) and anti-OVA IgG1 (g) measured at the end of the IntelliCage experiments. h–j, Absolute numbers of stomach mast cells (h), frequencies of small intestine intraepithelial mast cells (i) and small intestine lamina propria mast cells (j) among total live cells, measured at the end of the IntelliCage experiments. Bars represent mean values, and each dot corresponds to a single mouse. In c–j, Cpa3+/+ alum (n = 13 mice for c–h, n = 12 for i and n = 13 for j); Cpa3+/+ OVA-alum (n = 16 mice for c–h, n = 15 for i and n = 16 for j); Cpa3Cre/+ alum (n = 9 mice for c–h, n = 7 for i and n = 9 for j); Cpa3Cre/+ OVA-alum (n = 13 mice for c–h, n = 11 for i and n = 13 for j). Statistical analysis was performed using one-way analysis of variance with Tukey multiple-comparison test (e–j). Exact P values are shown. i.p., intraperitoneal.
