Fig. 4: In situ tracing of mast cell activation and role of FLAP in antigen avoidance.
From: Mast cells link immune sensing to antigen-avoidance behaviour
a, Model for detection of FcεRI-activated mast cells using the Nr4a1-GFP reporter allele. In vivo OVA-responsive mast cells expressed GFP. b, (BALB/c x C57BL/6)F1 Nr4a1-GFP mice immunized with OVA-alum or alum only were given drinking water with OVA or BSA (Methods). Representative histograms (for data shown in Extended Data Fig. 7a) of GFP expression in tissue mast cells isolated ex vivo after drinking OVA- or BSA-containing water for 3 h. c, Model displaying the generation of leukotrienes and FLAP inhibition by MK-886. d,e, BALB/c wild-type mice were immunized with OVA-alum (as in Fig. 1a), and, 1 h before the IntelliCage (d), or two-bottle (e) test, mice were treated with PBS or MK-886 (Methods). Preference is displayed as the fraction of egg white water intake over total water intake for 6, 12 or 24 h. Bars represent mean values, and each dot corresponds to a single mouse. Alum vehicle (n = 5 mice), alum MK-886 (n = 5), OVA-alum vehicle (n = 8), OVA-alum MK-886 (n = 12) (d), alum vehicle (n = 14), alum MK-886 (n = 14), OVA-alum vehicle (n = 19), OVA-alum MK-886 (n = 19) (e). Statistical analysis was performed by two-sided Student’s t tests in d and e. Exact P values are shown. AA, arachidonic acid. Figure 4a created with BioRender.com.
