Fig. 1: Cold-induced FRI–GFP condensate formation and FLC repression are attenuated in ΔCOOLAIR-1.
From: Reply to: Cold induction of nuclear FRIGIDA condensation in Arabidopsis
a–c, Quantification of the FRI–GFP nuclear condensate area (spot area (a) and percentage (c)) and number per nucleus (b) in the roots of FRI-GFP and FRI-GFPΔCOOLAIR-1 homozygous F3 lines. Plants were exposed to cold treatment for 4 days at a constant temperature of 5 °C. For a and b, the open circles indicate the median of the data and the vertical bars indicate the 95% confidence interval determined by bootstrapping. n = 1,729 and 2,548 condensates (a) and n = 271 and 529 (b) nuclei in n = 15 and 26 roots, respectively. Individual data points are shown as black or red dots. Comparison of mean values was performed using two-tailed t-tests with Welch’s correction. d,e, The relative expression level of unspliced FLC (d) and spliced FLC (e) in Col FRI, FRI ΔCOOLAIR-1 and FRI TEX1.0 plants with 2 weeks of growth under the indicated temperature conditions6. Data are mean ± s.e.m. of n = 6 biologically independent experiments. f, Schematic of FLC and COOLAIR transcripts at the FLC locus. Untranslated regions are indicated by grey boxes and exons by black boxes. Head-to-head arrows indicate primers used for antisense transcript level analysis by quantitative PCR (qPCR). g–i, The relative expression level of antisense transcripts at FLC, including but not limited to CAS6, by the indicated primers (P1 (CAS) (g), P3 (h) and P4 (i)) in Col-FRI and FRIΔCOOLAIR-1 plants with 24 h of growth under the indicated temperature conditions. The primers used are indicated in f. Data are mean ± s.e.m. of n = 3 biologically independent experiments. NS, not significant; the exact P values are shown at the top of each comparison.
