Extended Data Fig. 3: Biochemical analysis for NINJ1-anti-NINJ1 Fab complex.
From: Inhibiting membrane rupture with NINJ1 antibodies limits tissue injury
a, Schematic of the procedures in Fig. 3c,d,e, and Extended Data Fig. 3b, c, d. b, Coomassie blue staining of NINJ1 alone (retention volume 8.5-9 ml) and NINJ1-clone D1 Fab complex (retention volume 15-15.5 ml) of Fig. 3c or NINJ1-clone 25 Fab complex of (d) (retention volume 15.5-16 ml). Results representative of 2 independent experiments. c, Negative stain electron microscopy of NINJ1-clone 25 Fab complex. Results representative of 2 independent experiments. d, Size exclusion chromatography traces for purified NINJ1-clone 25 Fab complex. Results representative of 3 independent experiments. Molecular weight standard marker positions are indicated by arrows.
