Extended Data Fig. 5: Cryo-EM data processing flowchart for the BCDX2/ssDNA structure.
From: Structural insights into BCDX2 complex function in homologous recombination
Auto-picked particles were first sorted by three rounds of 2D classification. Next, six initial models were generated, followed by four rounds of heterogenous refinement. A subsequent step of stringent heterogenous refinement and tight mask homogeneous refinement of class 3 resulted in a map with 3.12 Å resolution. A final non-uniform refinement yields final map of 3.11Å resolution. All data processing was performed using Cryosparc v3.3.1.
