Extended Data Fig. 7: A potential mechanism for the ssDNA binding specificity of BCDX2.
From: Structural insights into BCDX2 complex function in homologous recombination
a, RAD51C from BCDX2 was superposed onto RAD51 from the RAD51/ssDNA postsynaptic structure (PDB: 7EJE). Three subunits from the RAD51 filament are presented. An overview of the superposition is shown in the center panel, while magnified views of the RAD51C/RAD51D and RAD51D/XRCC2 subcomplexes are shown to the right and left, respectively. b, Magnified view of the RAD51D/XRCC2 module from panel a, rotated 180 degrees about the y-axis (left). Right, dsDNA from the RAD51/dsDNA postsynaptic complex is shown as spheres with the RAD51 structure omitted for clarity. Note clashes between dsDNA and XRCC2 due to 11 and 23 degree tilts at the RAD51C/RAD51D and RAD51D/XRCC2 interfaces, respectively, compared to RAD51. c, Surface electrostatic representation of the BCDX2 complex with dsDNA from the superposition presented in panels a and b. Note clashes between dsDNA and XRCC2 and close proximity of the NTDs of BCDX2 to dsDNA. d, Superposition of XRCC2 onto RAD51. Note structural differences in length and conformation of the loop 1 region including helices H12 and H14, which clash with dsDNA in the model.
