Extended Data Fig. 5: Analysis of evoked GCaMP6m signals in VTA-DA neurons.
From: Circuit coordination of opposing neuropeptide and neurotransmitter signals
(a–b) Average GCaMP6m fluorescence (Z-score) during and after 1 s (a) and 3 s (b) stimulation at 40 Hz. Arrows indicate initial and final peaks at the onset and offset of the stimulation, respectively. (c) Average area under the curve (AUC) of the Z-scored GCaMP6m fluorescence during 1 s stimulation at different frequencies. (d) Average AUC for the 10 s following 1 s stimulation at different frequencies. (e) Average AUC during and after 1 s stimulation at different frequencies. (f) Average AUC during and after 3 s stimulation at different frequencies. (g) Average initial peak amplitude (Z-score) of GCaMP6m fluorescence during 1 s stimulation at different frequencies. (h) Average final peak amplitude (Z-score) of GCaMP6m fluorescence during 1 s stimulation at different frequencies. (i) Difference between final and initial peak amplitude (final-initial) of GCaMP6m fluorescence during 1 s stimulation at different frequencies. (j) Average initial peak amplitude (Z-score) of GCaMP6m fluorescence during 3 s stimulation at different frequencies. (k) Average final peak amplitude (Z-score) of GCaMP6m fluorescence during 3 s stimulation at different frequencies. (l) Difference between final and initial peak amplitude (final-initial) of GCaMP6m fluorescence during 3 s stimulation at different frequencies. (Control N = 8, sgVgat N = 7 sgNtsr1 N = 6, sgVgat+sgNtsr1 N = 5. *P < 0.05; **P < 0.01; ***P < 0.001; red asterisk sgVgat vs. control; blue asterisk sgNts vs. control; purple asterisk sgVgat + sgNtsr1 vs. control.) Data are presented as mean ± S.E.M.
