Extended Data Fig. 8: Minireplicon assays with avian/mammalian NP reassortant RNP complexes.

a, 293T-Empty and 293T-BTN3A3 cells were transfected with pcDNA plasmids encoding for PB2, PB1, PA and NP of the indicated viruses alongside firefly luciferase-coding vRNA- or cRNA-like reporter plasmids. A transfection control plasmid expressing Renilla firefly was also added. Forty-eight hours post-transection cells were lysed and firefly and Renilla luciferase activities were measured. Values were normalised to PR8 or Cal04 WT replicons with respective NPs transfected in 293T-Empty cells. Data are mean +/− SEM of 3 independent experiments (each using 2 technical replicates). Statistical differences between Empty and HsBTN3A3 overexpressing cells were calculated using multiple t-tests and corrected for multiple comparisons using the Holm-Šídák method. NS = non-significant, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. b. Expression levels of PB2, PB1, PA and NP transfected in 293T-Empty (E) or 293T-BTN3A3 (B3) cells were assessed by western blot. α-Tubulin was used as loading control. For gel source data, see Supplementary Fig. 1.