Extended Data Fig. 11: Distinct requirements of NP residues for BTN3A3 and Mx1 evasion, and GWAS analysis of H7N9 patients.
From: BTN3A3 evasion promotes the zoonotic potential of influenza A viruses
a, A549 cells overexpressing human Mx1 or BTN3A3 were infected with the mentioned viruses at a MOI of 0.001. Supernatants were harvested at 48 hpi and infectious viral titres were measured by plaque assay. Data are mean +/− SEM of 3 independent experiments (each using 2 technical replicates). Statistical significance between groups was measured by a 2-way ANOVA. NS- non-significant, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. (b-c) GWAS analysis of H7N9-infected patients. Manhattan plots of MX1 (b) and BTN3A3 (c) genomic regions. Gene-level p-values were acquired using RACER in R from data obtained from Chen, et al., Science 373, 918-922, doi:10.1126/science.abg5953 (2021).
