Extended Data Fig. 2: Evolution of BTNs.
From: BTN3A3 evasion promotes the zoonotic potential of influenza A viruses
a, Maximum likelihood phylogeny of concatenated protein domain coding sequences of the Haplorrhini BTN3 genes (K2P+G4 substitution model). Nodes with bootstrap support below 60 have been collapsed. Branches confirmed to have or not have anti-avian IAV activity (described in b) are highlighted in yellow and blue, respectively. Branches not tested were kept black. Relation to more distant tested homologues and orthologous/paralogous gene families are shown as a schematic in grey. IgV homogenization events, major gene duplications and gene subfamilies are annotated on the phylogeny. Presence of each of the four protein domains (IgV, IgC, PRY and SPRY) is annotated on the right of each tree tip and coloured by pairwise amino acid similarity to the respective domain of the human BTN3A3. Species names and taxonomic classification is annotated on the right. The median divergence time between Catarrhini and Platyrrhini was retrieved from TimeTree (http://timetree.org/). b, Schematic representation of domain organisation and sequence similarity of the indicated proteins.
