Extended Data Fig. 3: Evolution of antiviral activity of BTNs.
From: BTN3A3 evasion promotes the zoonotic potential of influenza A viruses
a, A549 cells were transiently transduced with SCRPSY lentiviruses expressing the indicated BTN proteins and challenged with PR8- or Mallard-GFP. Eight hours post infection, the percentage of GFP-positive cells was measured by flow cytometry. Data are mean +/− SEM of 2 independent experiments which both gave similar results. b, Western blotting of cell lysates obtained from A549 cells transiently transduced with SCPRSY lentiviruses expressing different BTN proteins. GAPDH was used as loading control. For gel source data, see Supplementary Fig. 1.
