Extended Data Fig. 3: Gut microbial profiling and CLA quantification of rich-diet versus minimal-diet mice.
From: Gut microbial fatty acid isomerization modulates intraepithelial T cells
(a–e) Three-week-old SPF mice were fed a nutrient-rich diet or a minimal diet, and the microbial compositions in the ileal and colonic lumen were analyzed after 4 weeks by 16S rRNA sequencing. PCoA analysis on ileal (a) or colonic (b) luminal content, Rank abundance curve (c), and relative abundance of bacteria at the phylum (d) and family levels (e) are shown. (f) GC/MS quantitation of different CLAs in the luminal contents from the duodenum, jejunum, and ileum from SPF mice fed a nutrient-rich diet or a minimal diet. (g) LC/MS quantitation of CLAs in the luminal contents from the duodenum, jejunum, and ileum from SPF or GF mice. (h) LC/MS quantitation of CLAs produced by the indicated gut commensal strains cultured with linoleic acid (60 μM) for 24 h. Data are pooled from two independent experiments in a–e. Data are representative of two independent experiments in f–h. n represents biologically independent animals in a–c and f–g, and biologically independent samples in h. Bars indicate mean ± SEM values. Statistical analysis was performed using a two-tailed Student’s t-test in f and g.
