Extended Data Fig. 10: Continuous synthesis of 500 kb of a recoded E. coli genome from CONEXER BACs.
From: Continuous synthesis of E. coli genome sections and Mb-scale human DNA assembly
Continuous genome synthesis via rounds of CONEXER in ∆recA recipients. Genomic DNA is depicted in grey and synthetic, recoded DNA in pink. The selectable markers are +1, blue, kanR (selected for with kanamycin); −1, yellow, rpsL (selected against with streptomycin); +2, green, cat (selected for with chloramphenicol); −2, pink, sacB (selected against with sucrose). Each round of CONEXER replaces approximately 100 kb of the E. coli genome with synthetic DNA, and takes two days. Continuous synthesis of a 500 kb synthetic section in the E.coli genome was achieved in 10 days.
