Extended Data Fig. 4: oviDN [Ca²⁺] traces from individual egg-laying events.

a, OviDN ∆F/F showing all individual traces that were averaged in Fig. 1h (43 imaging traces from 41 egg-laying events associated with 9 cells in 8 flies). Grey lines are individual traces smoothed with a 5 s boxcar filter. Black line is the average of the non-smoothed individual traces. b, Each row represents a single egg-laying event. Rows have been ordered based on the search duration. Individual traces are smoothed as in panel a and behavioral annotations are overlayed. Individual traces corresponding to Fig. 1g and Fig. 1m (fly 3) are highlighted. Individual eggs that are part of the analysis in Fig. 4e, f are marked with an asterisk. c, Same as panel b but with a colormap where white is centered on a ∆F/F of 0 which is the average baseline in our normalization (Methods). Individual traces tend to (1) dip below baseline during ovulation (blue color after light pink/magenta line); (2) return to baseline around the time of search start (white color near dark pink/purple line); and (3) increase past baseline around or after the search start (red color after dark pink/purple line). These trends are captured by the average analysis presented in Fig. 1j–l. d, Normalized oviDN ∆F/F showing all individual traces in Fig. 1h (43 imaging traces from 41 egg-laying events associated with 9 cells in 8 flies). Grey lines are individual traces smoothed with a 5 s boxcar filter and then normalized such that the maximum and minimum ∆F/F in the 100 s window preceding the abdomen bend are set to 1 and 0, respectively. Black line is the average of the smoothed individual traces. e, Same as panel b but displaying ∆F/F normalized as in panel d.