Extended Data Fig. 3: Anatomy and physiology of different oviDN types.

a, Electron-microscopy (EM) skeletons¹⁶ and characterization of the 3 oviDN and 2 oviDN-like neurons per side. The branch labeled in grey is sometimes present in oviDNb⁹ and sometimes not (Fig. 1e). The 3 other arrows indicate neurites that are unique to either oviDNa or oviDNb. Visualization generated using Neuroglancer. Neuropil to left is only to schematize the approximate ROI shown in the EM. b, Average z-projection of oviDN-GAL4 in the brain (top) and ventral nerve cord (bottom). Green shows UAS-mCD8GFP expression in the targeted neurons and magenta represents a neuropil counterstain (Methods). c, Anatomy of oviDN-SS1 driving expression of GCaMP7f. The brighter of the two oviDN cell bodies was filled with Texas Red (Methods). The neurite labeled with a pink arrow in panel a was used to determine if the cell was oviDNb. All 6 of the brighter cells filled with Texas Red (from 6 separate flies) were oviDNb. Two examples are shown (representative individual z-slices). d, Mean oviDNa ∆F/F during individual egg-laying events. 29 traces from 7 cells in 6 flies (28 eggs). These data did not contribute to the traces in Fig. 1 (or any other figure), which were exclusively from oviDNb. Light grey shading is ± s.e.m. for all panels in this figure. e, Mean cross-correlation of ∆F/F between ipsilateral oviDNa and oviDNb cells imaged simultaneously. Traces from multiple, individual cell pairs are averaged. f, Mean cross-correlation of ∆F/F between contralateral oviDNb cells imaged simultaneously. Traces from multiple, individual cell pairs are averaged.