Extended Data Fig. 9: Treg co-transplantation suppresses C4-mDAP proliferation in a xenogeneic model of PD.
From: Co-transplantation of autologous Treg cells in a cell therapy for Parkinson’s disease
a–k, Fischer 344 rats were killed 20 weeks after intra-striatal co-transplantation of C4-mDAPs with or without Treg cells and CsA. a,b, Stereological estimation of graft volume through hNCAM+ staining. Number of Ki67+ cells (c,d) among hNUCLEI+ cells. (Two-tailed unpaired t-test; n = 5 per group). e, Correlation between Ki67+ cell number and graft volume. (Two-tailed correlation). f,f′, The number of TH+ and FOXA2+ cells was analysed by immunofluorescence staining. (Two-tailed unpaired t-test; Mann–Whitney post-hoc test; n = 20 per group). g–k, Representative images of neuronal cells (g, NeuN+), astrocytes (h, hGFAP+), vascular leptomeningeal cells (i, hCOL1A1+), oligodendrocyte lineage cells (j, OLIG2+) and microglia (k, hIBA1+). (5 rats per group were identified). In box plots in b,d,f′, the centre line represents the median, box edges represent the interquartile range, and error bars represent maximum and minimum values. Scale bars: 100 μm. Each error bar represents mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001.
