Extended Data Fig. 1: Influence of needle-trauma-induced neuroinflammation on transplanted post-mitotic neurons.
From: Co-transplantation of autologous Treg cells in a cell therapy for Parkinson’s disease
a–e, NSG mice were killed 14 days after intra-striatal transplantation of B1/H9-mDAPs. The total number and percentage of TH+ mDANs were determined by immunofluorescence staining with anti-TH/Hoechst 33342 before transplantation (a,c,e) or immunohistochemistry staining with anti-TH/hNUCLEI antibodies after transplantation (b,d,e). (n = 4 per group). f–i, NSG mice were killed 14 days after intra-striatal transplantation of C4-iPS cell/B1-iPS cell/H9-ES cell-derived GABAergic progenitors. The total number and percentage of C4- (f), B1- (g) or H9- (h) GABAergic neurons were determined by immunofluorescence staining with anti-VGAT/Hoechst 33342 before transplantation and anti-VGAT/NKX2.1/hNUCLEI antibodies after transplantation (i). (n = 4 per group). Each error bar represents mean ± s.e.m.
