Extended Data Fig. 6: Cell biology of PLSCR1 in anti-SARS-CoV-2 activity.
From: PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection
a, Representative images showing the localization of endogenous PLSCR1 and SARS-CoV-2 spike in WT A549-ACE2 cells during the early stage of virus entry. Time post-infection is indicated. (MOI = 25). b, Representative images showing the localization of endogenous PLSCR1 and SARS-CoV-2 nucleocapsid in WT A549-ACE2 cells during the early stage of virus entry. Time post-infection is indicated. (MOI = 25). c, Deconvolution confocal images showing the colocalization of endogenous PLSCR1 and SARS-CoV-2 nucleocapsid. (MOI = 25, 2 hpi). d, Three-dimensional confocal microscopy images showing the colocalization of endogenous PLSCR1 and SARS-CoV-2 nucleocapsid in A549-ACE2 cells at 2 hpi. (MOI = 25). e, Representative images of the localization of endogenous PLSCR1 and viral replication centre (indicated by dsRNA) in A549-ACE2 cells during virus infection. Time post-infection is indicated. (MOI = 25). f, Representative images showing the localization of endogenous PLSCR1 and human transferrin–Alexa Fluor 488 30 min after treatment. Scale bar in a,b,e: 10 μm, inlays: 5 μm. Scale bar in c: 5 μm, inlays: 1 μm. Scale bar in d,f: 20 μm, inlays: 5 μm. Experiments in this figure were performed three times, except a–c (five times).
