Extended Data Fig. 5: Mammary duct ablation potentiates the beiging of mgWAT.
From: Mammary duct luminal epithelium controls adipocyte thermogenic programme
a) Whole tissue images and H&E sections of mgWATs of WT and Esr1KO cold exposed mice. Blue asterisks represent same samples at different magnifications. Scale bar at 100 μm. b and c) Cartoon showing intraductal injection into the 4th and 5th nipples of female mice (b) with adjacent cartoon showing injection of Evan’s blue or GFP expressing virus for confirmation ductal-specific injection (c). Scale bar at 100 μm d) Intraductal injection of Krt8-Dtr mice with contralateral injection of DTA or PBS and immunohistochemistry with DAPI or UCP1. White arrows indicated UCP1 signals in the stroma and yellow arrows indicated UCP1 signal in ducts. Scale bar at 50 μm e) H&E staining of mgWAT from mice intraductally injected with PBS, 70% EtOH, or 0.5% Trypsin. Yellow arrows indicate ablated ducts. Red asterixis indicate images used in Main Fig. 3j. Representative section from 7 mice. Scale bar at 50 μm. f) Immunofluorescence microscopy for UCP1 or DAPI in mice intraductally injected with PBS or EtOH. Dotted yellow outlines are ductal epithelium. Scale bar at 100 μm. g) Real-time qPCR analysis of indicated genes from mgWATs of mice intraductally injected with PBS or Trypsin. N = 9,9. Data are represented as mean ± S.E.M. Cartoon models in b,d,g created using BioRender.
