Extended Data Fig. 5: Post-implantation human embryo-like model cluster identification.
From: Pluripotent stem cell-derived model of the post-implantation human embryo
a, Day 4 embryoids generated from a second hESC line, RUES2. N = 371 structures from 2 independent experiments. b, Brightfield image of inducible human embryoids selected at days 4, 6, and 8 for sequencing (n = 12 at each stage). Note the presence of an inner domain surrounded by two concentric domains. c, Quality control plots for embryoid sequencing data at days 4, 6, and 8 post-aggregation. n = 5217 cells. Violin plots go from minimum to maximum. b, scmap projection of inducible human embryoid cells onto cynomolgus macaque (M. fasicularis) and human datasets (H. sapien) spanning peri-implantation to gastrulation stages. e, Cardinal marker gene expression for epiblast, endoderm and mesoderm, and trophoblast and amnion within the stem cell-derived model. f, Alluvial plot showing the contribution of day 4, 6, or 8 embryoids to assigned cell type. Scale bars = 100 µm. Inner domains of embryoids are surrounded by a dashed line.
