Extended Data Fig. 3: Comparison of transcription factor-mediated induction with published directed differentiation methods.
From: Pluripotent stem cell-derived model of the post-implantation human embryo
a, Comparison and quantification of GATA6, SOX17 and SOX2 after yolk sac-like cell (Activin-A, CHIR99021 and LIF) directed differentiation, doxycycline-mediated induction in inducible GATA6-SOX17 cells, or both. Cells were differentiated from RSeT conditions. b, Comparison and quantification of EOMES, N-Cadherin, and OTX2 after yolk sac-like cell (Activin-A, CHIR99021, and LIF) directed differentiation, doxycycline-mediated induction in inducible GATA6-SOX17 cells, or both. For a-b, N2B27: n = 717; ACL: n = 1211; N2B27+Dox.: n = 522; and ACL+Dox.: n = 544 cells from 3 fields of view from 2 independent experiments. c, Comparison and quantification of GATA3, AP2α, and SOX2 after PA (PD0325901 and A83-01) or PAL (PD0325901, A83-01, and LPA) directed differentiation, doxycycline-mediated induction in inducible GATA3-AP2γ cells, or both. d, Comparison and quantification of GATA2, KRT7, and AP2γ after PA (PD0325901 and A83-01) or PAL (PD0325901, A83-01, and LPA) directed differentiation, doxycycline-mediated induction in inducible GATA3-AP2γ RseT cells, or both. For c-d, N2B27: n = 443; PA: n = 487; PAL: n = 371; N2B27+Dox.: n = 357; PA+Dox.: n = 412; and PAL+Dox.: n = 287 cells from 3 fields of view from 2 independent experiments. Scale bars = 100 µm. For a-d, mean ± SEM is plotted. Differentiation was carried out on hESC in RSeT conditions.
