Extended Data Fig. 6: PR migration behaviour and apical occupancy.
From: Neuronal migration prevents spatial competition in retinal morphogenesis
a, Correlation between maximum basal position and duration of PR migration. Dots represent individual cells (n = 49 cells, N = 17 embryos). b, Proliferative status of the retina during PR migration at 42 hpf. Tg(crx:gap-CFP) (magenta) labels PRs; EdU (yellow) labels cells in S-phase. c, Scheme of FACS sorting of PRs and bipolar cells. d, Box plots of the percentages of PRs and bipolar cells in the cell population negative for ptf1a:Gal4/UAS:gap-YFP measured by FACS (experiments = 6, retinas per sample = 25, minimum live cells sorted per experiment = 10,000, total live events sorted = 107,361). The lower and upper hinges of the box indicate the 25th and 75th percentile, respectively; middle line indicates the median, whiskers indicate minimum and maximum. Dots represent individual measurements. e, Co-expression of crx and ath5 promoter-driven reporters at 42 hpf. Tg(crx:gap-CFP) (magenta) labels PRs; Tg(ath5:gap-RFP) (cyan) labels RGCs, PRs and neurogenic progenitors. Dashed white boxes indicate areas shown in e’. Yellow dots mark PRs. f, Measurement of apical surface area of retinal tissue at 42 hpf using the Fiji plug-in Volume Manager. Tg(crx:gap-CFP) (grey) labels PRs. Yellow grid marks the apical surface. g, Measurement of the cross-sectional area of apical PRs at 42 hpf. Tg(crx:gap-CFP) (grey) labels PRs. Yellow line in the XY view outlines the plane shown in XZ view (g’). h, Abundance of basal PRs at 42 hpf. Tg(crx:gap-CFP) labels PRs, colour represents z depth, lookup table shows minimum and maximum z depths. i, Progenitor movements and apical mitosis after colcemid treatment in an area in which PRs had not yet emerged. Embryos were treated with 25 μM colcemid. Tg(crx:gap-CFP) in magenta labels PRs; hsp70:H2B-RFP in yellow labels apically migrating nuclei. Time is displayed as hours:minutes. Blue and white dots mark progenitor cells; arrowheads mark apical mitoses. Scale bars: 10 μm (g,i), 15 μm (b), 20 μm (f,e,h).
