Fig. 1: Mitochondrial complex I in lung epithelial cells is necessary for postnatal lung development.
From: Mitochondrial integrated stress response controls lung epithelial cell fate
a, Schematic of the mitochondrial ETC in lung epithelial cells of NDUFS2 cKO mice. b, Immunoblot analysis of NDUFS2 protein normalized to vinculin in lung epithelial cells isolated from 11-day-old mice. Data represent mean ± s.d. (n = 4 mice in each genotype with technical replicates). c, Basal and coupled OCR of lung epithelial cells isolated from 43- to 46-day-old mice. Data represent mean ± s.d. (Ndufs2fl/fl n = 3; NDUFS2 control n = 4; NDUFS2 cKO n = 5 mice with technical replicates). d, Body weight in grams (control n = 34; cKO n = 18 mice). Data represent mean ± s.d. **P = 0.0040, ***P = 0.0005 by Mann–Whitney test. e, Survival of NDUFS2 control (n = 21) and NDUFS2 cKO (n = 13) mice (P < 0.0001 by log-rank test). f, Representative images of lung histology on postnatal day 49 (haematoxylin and eosin stain). Scale bar, 100 μm. g, The frequency distribution of alveolar thickness measured in haematoxylin and eosin-stained lung histology of 46- to 48-day-old mice (n = 4 mice, two males and two females per genotype). Four to six randomly selected fields of view from each mouse were evaluated. The x axis shows alveolar thickness bins and the y axis shows the number of alveolar pixels that belong to the respective alveolar thickness bin normalized to the total alveolar pixel count in the image. Each animal is represented by its own colour. Statistical significance for genotype was calculated based on F-test for a linear model (P = 4.56 × 10−5). h, Box plots of lung compliance in 46- to 49-day-old mice (control n = 33; cKO n = 24 mice with technical replicates), P < 0.0001 by Mann–Whitney test.
