Extended Data Fig. 10: Differentiation of hiPSCs.
From: Transient naive reprogramming corrects hiPS cells functionally and epigenetically
a) Representative immunofluorescence analysis images of cell differentiation: SOX1 and PAX6 for cortical neuron differentiation; GATA6 and TTF1 for lung epithelial differentiation; PAX3 and PAX7 for skeletal muscle differentiation. Scale bar: 50 μm. b) Representative flow cytometric profile of cell differentiation: CXCR4/SOX17 for endoderm differentiation; CD56/CD57 for cortical neuron differentiation; CD47/EPCAM for lung epithelial differentiation; and CD56/CD146 for skeletal muscle differentiation. c) Representative immunofluorescence analysis images of cell differentiation: SOX17 and FOXA2 for endoderm differentiation; SOX1 and PAX6 for cortical neuron differentiation; GATA6 and TTF1 for lung epithelial differentiation; PAX3 and PAX7 for skeletal muscle differentiation. Scale bar: 50 μm. d) Representative flow cytometric profile of cell differentiation: CXCR4/SOX17 for endoderm differentiation; CD56/CD57 for cortical neuron differentiation; CD47/EPCAM for lung epithelial differentiation; and CD56/CD146 for skeletal muscle differentiation. Replicate details of the differentiation experiments can be found in the ‘Statistic and reproducibility’ section in Methods.
