Extended Data Fig. 6: Obox depletion impaired ZGA and MERVL activation.
From: OBOX regulates mouse zygotic genome activation and early development
a, Hierarchical clustering based on RNA-seq (2 biological replicates for E2C and 3 for L2C). b, Volcano plot showing gene expression changes upon Obox depletion (2 biological replicates for E2C and 3 for L2C). Dashed line, adjusted P-value threshold 0.05. GO terms are shown. c, Balloon plot showing gene expression changes (mzKO/WT) for MERVL and ZGA genes at 2C (2 biological replicates for E2C and 3 for L2C). d, Scatter plot showing gene expression fold-changes upon Obox depletion (2 biological replicates for E2C and 3 for L2C). FC, fold-change. Yellow lines, local regression fitting. e, Violin plot showing maternal and ZGA gene expression changes from oocytes to E2C or L2C in WT and Obox mzKO embryos (2 biological replicates for MII, E2C and 3 for L2C). Centre line, median; box, 25th and 75th percentiles; whiskers, 1.5 × IQR.
