Extended Data Fig. 10: OBOX activated ZGA genes in mESCs independent of DUX and NR5A2.
From: OBOX regulates mouse zygotic genome activation and early development
a, Bar charts showing Dux, Zscan4, and Dppa expression in 2C embryos (top, 2 biological replicates for E2C and 3 for L2C) and mESCs (bottom, 4-5 biological replicates). b, The UCSC browser snapshots showing OBOX binding at 2C. Pol II, ATAC, and OBOX motif are shown. c, Heatmap showing Obox expression upon Dux overexpression16 in 2i mESCs (2 biological replicates). d, Heatmap showing Obox expression upon Dux knockout20 (2 biological replicates for late 1-cell (L1C) and 3 for L2C). e, Venn diagram showing the overlap of downregulated ZGA genes between Obox knockout and Dux knockout embryos19,20. n, ZGA gene number. f, Venn diagram showing the overlap of OBOX-activated ZGA genes and upregulated ZGA genes in 2CLCs compared to mESCs. 2CLC, 2-cell-like cell. P-value, two-sided Fisher’s exact test. Green indicates the combined ZGA gene list activated by OBOX3/5 and in 2CLCs. g, Heatmap showing Obox expression upon Nr5a2 knockdown21 in embryos. h, Scatter plot comparing the ZGA gene expression changes upon Obox overexpression between WT and Nr5a2 knockout mESCs (2 replicates).
