Extended Data Fig. 12: SBP and TPRL are present in human stool samples.
From: Mapping the T cell repertoire to a complex gut bacterial community
We tested whether SBP and TPRL exist at a detectable level in human stool samples. We cocultured SBP- or TPRL-reactive TCR hybridomas with: (1) PBS as a negative control, (2) a mix of SBP and TPRL epitopes as a positive control, (3) heat-treated hCom1d, (4) heat-treated SPF mouse fecal pellets, 5) heat-treated mouse fecal pellets from Jackson, (5) 6 human fecal communities. For antigen presentation, we used MHCII+ wild-type DCs, or MHCII-deficient DCs as a negative control. IL-2 concentration was measured as a readout for TCR stimulation. We found that 6/6 human fecal communities restimulate the SBP405-413 and SBP76-84-specific hybridomas and 5/6 restimulate the TPRL29-53-specific hybridomas. These data suggest that the SBP and TPRL are expressed by human gut isolates under native conditions.
