Fig. 1: paCaMKII photoactivation-induced sLTP requires GluN2B binding.
From: LTP induction by structural rather than enzymatic functions of CaMKII
Data are presented as mean values ± s.e.m. a, Schematic of how photoactivation of paCaMKII allows access to both S and T sites, thereby enabling both enzymatic CaMKII activity and GluN2B binding. b, Representative confocal microscopy images of HEK293 cells co-expressing GFP–paCaMKII (WT, K42M or I205K) and pDisplay-mCherry-GluN2B-c tail (mCh-2BC) before and after photoactivation. Scale bar, 10 µm. Quantification is shown in panel c. c, Pearson’s correlation (correlation index) of GFP–paCaMKII WT or mutants and mCh-2BC 5 min after photoactivation (n = 15, 20, 21 cells; one-sample t-test). WT P < 0.0001; K42M P = 0.9468; I205K P = 0.5885. ***P < 0.001. d, Representative confocal microscopy images of dissociated rat hippocampal cultures expressing GFP–paCaMKII (WT, K42M or I205K) and mCherry cell fill before and after photoactivation. Scale bar, 5 µm. Quantification is shown in panel e. e, Fold change of paCaMKII WT and mutant synaptic enrichment values 15 min after photoactivation (n = 11, 9, 9 cells; one-sample t-test). WT P = 0.0042; K42M P = 0.0568; I205K P = 0.3523. **P < 0.01; f, Fold change of paCaMKII synaptic enrichment values 15 min after photoactivation in WT and GluN2BΔCaMKII mice (n = 9, 9 cells; one-sample t-test). WT P = 0.0038; GluN2BΔCaMKII P = 0.2172. **P < 0.01. g, Changes in the dendritic spine area were measured after paCaMKII photoactivation via mCherry cell fill fluorescence intensity within a spine (n = 12, 8, 9, 9, 9 cells; one-sample t-test). In the left panel, WT P = 0.008; K42M P = 0.0692; and I205K P = 0.1236. In the right panel, WT P = 0.0481 and GluN2BΔCaMKII P = 0.1406. *P < 0.05, ***P < 0.001. NS, not significant.
