Extended Data Fig. 3: No difference in LTP in WT mice when CaMKII is inhibited by either 10 or 30 μM AS283. Data are presented as mean values +/− SEM.
From: LTP induction by structural rather than enzymatic functions of CaMKII
a, 2x HFS potentiates the CA3-CA1 Schaffer collateral pathway in WT mice when CaMKII enzymatic activity is inhibited by 10 or 30 μM AS283 (incubated for 15 min prior to HFS, and washed out 5 min after). b, Quantification of LTP in WT mice after 10 or 30 μM AS283 (n = 3,7 hippocampal slices; Mann-Whitney test). p = 0.3833. c, Representative immunoblot and quantification of CaMKII T286 phosphorylation in dissociated hippocampal cultures after cLTP and inhibition by 10 μM AS283 (n = 4 independent cell preparations; one-way ANOVA, Tukey’s multiple comparisons test). The inhibition of T286p in seen hippocampal cultures matches the inhibition seen in vitro (see Fig. 2b). Vehicle:cLTP p < 0.0001; Vehicle:cLTP+AS283 p = 0.0368; cLTP:cLTP+AS283 p = 0.0009. d, Representative immunoblot and quantification of in vitro kinase reactions at 30 °C with purified CaMKII and GST-2BC measuring S1303 phosphorylation and inhibition by 10 μM AS283 (n = 4 independent samples; one-way ANOVA, Tukey’s multiple comparisons test). –:ATP p < 0.0001; –:ATP+AS283 p = 0.0363; ATP:ATP+AS283 p = 0.0002. The inhibition seen for S1303 here matches the inhibition seen for T286 in hippocampal neurons in panel c.
