Extended Data Fig. 1: Evaluation of human ACE2 binding to SARS-CoV-2 variant RBDs.
From: Neutralization, effector function and immune imprinting of Omicron variants
a, Biolayer interferometry binding curves obtained for monomeric human ACE2 binding to biotinylated Wu, BA.4/5, BA.2.75.2, BQ.1.1, XBB.1 or XBB.1.5 RBDs immobilized at the surface of streptavidin biosensors. Kinetic rate constants and affinities are shown in Supplementary Table 1. Fits are shown as solid black lines. b, Sensorgrams of monomeric human ACE2 binding to the Wu, BA.2.75.2, BA.4/5, BQ.1.1, XBB.1, XBB.1.5 and Wu E340A RBDs immobilized at the surface of an SPR chip coated with anti-Avi polyclonal Ab. Experiments were performed with serial dilutions of Fabs and run as single-cycle kinetics. Gray blocks denote the dissociation phase. Fits are shown as dashed grey lines. Kinetic rate constants and affinities are shown in Supplementary Table 2.
