Extended Data Fig. 2: CryoEM data processing of the BQ.1.1, XBB.1 and BN.1 RBDs bound to ACE2 and S309.
From: Neutralization, effector function and immune imprinting of Omicron variants
a-b, Electron micrographs representative of 6,487, 6,355, or 3,822 micrographs, respectively, (a) and 2D class averages (b) of the BQ.1.1 RBD (left), the XBB.1 RBD (middle) or BN.1 RBD (right) bound to the human ACE2 ectodomain and the S309 Fab fragment embedded in vitreous ice. The scale bar represents 100 nm (a) or 100 Å (b). c-d, Gold-standard Fourier shell correlation curves (c) and local resolution maps along with angular distribution heat maps calculated using cryoSPARC (d) for the 3D reconstructions of the BQ.1.1 RBD (left), the XBB.1 RBD (middle) or BN.1 RBD (right) bound to the human ACE2 ectodomain and the S309 Fab fragment. The 0.143 cutoff is indicated by a horizontal dashed line. e, Data processing flowchart. CTF: contrast transfer function; NUR: non-uniform refinement.
