Extended Data Fig. 7: Optogenetic activation of VLS CINs, and histology for TelC expression in VLS CINs.
From: Dopamine and glutamate regulate striatal acetylcholine in decision-making
a, Ach release during optogenetic activation of Chrimson-expressing CINs. Average ∆F/F0 ± s.e.m. of Ach3.0 is depicted (laser stimulation artefacts omitted). b, Injection and optical fibre implantation for mice recorded in e. c, Images of dLight1.1, mCh, and Chrimson expression in the VLS for a representative mouse recorded in e. Coronal sections show spread of expression. A higher-resolution image of the recording site (dashed box) is shown with the fibre tract denoted (inset). Scale bars (white): 1 mm; (orange): 0.2 mm. d, Location of the optical fibre tip (pink dot) for mice recorded in c. e, DA signals during optogenetic activation of CINs. Average dLight1.1 ∆F/F0 ± s.e.m. is depicted (n = 7 mice). Laser stimulation artefacts are omitted. Quantification of the change in peak dLight1.1 signal from baseline is shown, with each connected pair of dots representing a unique mouse. Significance is calculated from a two-sided t-test (P = 0.0008). f, The decision time and side-port occupancy duration of mice with or without stimulation of the indicated hemispheres (n = 7 mice). Data are shown as in e. None of the comparisons are significant (two-sided t-test; P > 0.05). g, Images of Ach3.0 and mCh expression for a representative mouse recorded for Fig. 3d. Images are depicted as in c. h, Location of the optical fibre tip (pink dot) for mice recorded in Fig. 3d. i, Images of Ach3.0 and TelC expression for a representative mouse recorded for Fig. 3d. Histology is depicted as in c. j, Location of the optical fibre tip (pink dot) for mice recorded in Fig. 3d. k, DA kernels for the base GLM derived from Fig. 3e. The average kernel ± s.d. is shown. l, Images of dLight1.1 and striatum-wide mCh expression for a representative mouse recorded for Fig. 3e. Histology is depicted as in c. m, Location of the optical fibre tip (pink dot) for mice recorded in Fig. 3e. n, Images of dLight1.1 and striatum-wide TelC expression in the VLS for a representative mouse recorded for Fig. 3e. Histology is shown as in c. o, Location of the optical fibre tip (pink dot) for mice recorded in Fig. 3e.
