Extended Data Fig. 8: Histology, photometry recordings and analysis of the effects of CIN-specific TelC expression in the VLS on DA signals.
From: Dopamine and glutamate regulate striatal acetylcholine in decision-making
a, Images of dLight1.1, mCh, and TelC expression in the VLS for a representative mouse recorded for Fig. 3f. Scale bar (white): 1 mm. b, Images of dLight1.1, mCh, and TelC expression for a representative mouse recorded for Fig. 3f in which mCh and TelC expression is amplified through antibody staining. Coronal sections show spread of expression. A higher-resolution image of the recording site (dashed box) is shown with the fibre tract denoted (inset). Scale bars (white): 1 mm; (orange): 0.2 mm. c, Location of the optical fibre tip (pink dot) for mice recorded in Fig. 3f. d, Effect of TelC or mCh expression in CINs on DA release parsed by the action-outcome history of the previous trial (mean z-score ± s.e.m.; n = 4 mice). e, DA kernels for the history GLM derived from recordings in Fig. 3f with VLS expression of the indicated proteins in CINs. The average kernel ± s.d. are shown. f, Effect of TelC or mCh expression in CINs on DA release. ∆F/F0 signals for individual mice and the average ∆F/F0 ± s.e.m. are shown (n = 4 mice). g, Simultaneous recordings of Ach and DA from mice with VLS CINs expressing TelC or mCh (mean z-score ± s.e.m.; n = 4 mice). h, Effect of TelC expression in CINs on DA transients from g parsed by action-outcome history of one trial back. Data are depicted as in d. i, Analysis of DA signals from mice in g and Fig. 3f. Paired comparisons are shown between DA signals recorded from hemispheres with CINs expressing TelC (left dot in connected pair) or mCh (right dot in connected pair). The mean signal ± s.d. are shown. Data are depicted as in Extended Data Fig. 3a. Open circles represent a significant difference for each comparison (two-sided t-test (P < 0.05)). j, Analysis of DA signals from mice recorded in g and Fig. 3f. Data are depicted as in i. k, Analysis of DA signals from mice recorded in g and Fig. 3f parsed by the indicated action-outcome one, two and three trials back. Data are shown as in i.
