Extended Data Fig. 11: Photometry, GLM and behavioural analyses of DA and Ach signals from mice lacking D2R expression in CINs.
From: Dopamine and glutamate regulate striatal acetylcholine in decision-making
a, Ach and DA release from mice in Fig. 4g (mean ± s.e.m.; WT: n = 12 mice; Drd2 f/f: n = 7 mice; Drd2-cKO: n = 8 mice). b, Kernels for the history GLM of Ach signals from mice in Fig. 4g. Average kernels ± s.d. are depicted. c, Kernels for the history GLM of rDAh signals from mice in Fig. 4g. Data are displayed as in b. d, Statistical analysis of DA and Ach signals in Fig. 4g. Schematic of the time periods for analysis (left). Comparisons are shown as mean ± s.d. between the indicated genotypes (WT: ChAT-Cre; FF: Drd2 f/f; KO: Drd2-cKO). Open circles denote a significant difference across the three genotypes for a particular condition (two-sided ANOVA, open circle, P > 0.05). A two-sided t-test (P < 0.05) confirmed that KO signals were significantly different from FF and WT. e, Time shifts and amplitudes of the minimum covariance in Fig. 4h. Bars denote mean with 95% C.I. Significance is calculated using a two-sided t-test (P < 0.05, asterisk; values reported in Supplementary Data 1). f, Performance metrics for mice in Fig. 4g. Each dot represents a unique mouse. Bars denote mean with 95% C.I. No significant differences were observed (two-sided t-test, P >0.05). g, Probability of occupancy at the highly rewarded port (mean ± s.e.m.) for mice in Fig. 4g. Calculated taus are shown (mean with 95% C.I.), with each dot representing a unique mouse. h, Probability of switching (mean ± s.e.m.) for mice in Fig. 4g. Average maximum P(switch) rates are shown with 95% C.I., with each dot representing a unique mouse. i, RFLR coefficients for mice in Fig. 4g. Bars denote mean with 95% C.I. Significance is determined using a two-sided Wilcoxon rank-sum test (*P: β = 0.014; τ = 0.018). j, Conditional switch probabilities for the indicated action-outcome sequence for mice in Fig. 4g. Bars show the mean switch probability with the binomial standard error for the mouse test data. Significance (asterisk) is denoted as >95% C.I. from bootstrapped samples (one-sided, no adjustments).
