Extended Data Fig. 13: Histology for cortical inhibition, and behavioural effects of cortical and thalamic inhibition.
From: Dopamine and glutamate regulate striatal acetylcholine in decision-making
a, Images of Ach3.0 and mCh expression for a representative mouse recorded for Fig. 5f. Coronal sections show the spread of expression. A higher-resolution image of the recording site (dashed white box) is shown with the fibre tract denoted. Scale bars (white): 1 mm; (orange): 0.2 mm. b, Location of the optical fibre tip (pink dot) for mice recorded in Fig. 5f. c, Images of Ach3.0 and TelC expression for a representative mouse recorded for Fig. 5f. Histology is depicted as in a. d, Location of the optical fibre tip (pink dot) for mice recorded in Fig. 5f. e, Effect of TelC or mCh expression in Vglut2-Cre or Vglut1-Cre mice on Ach release (mean ∆F/F0 ± s.e.m.). f, Performance metrics for mice in Fig. 5e. Each dot represents a unique mouse. Bars denote mean with 95% C.I. Significance is determined by a two-sided t-test (*P: reward rate = 0.022; win-switch = 0.025). g, Probability of occupancy at highly rewarded port or switching (mean ± s.e.m.) for mice in Fig. 5e. Calculated taus and maximum P(switch) rates are shown (mean with 95% C.I.) with each dot representing a unique mouse. h, Conditional switch probabilities for the indicated action-outcome sequence for mice in Fig. 5e. Bars show the mean switch probability with the binomial standard error for the mouse test data. Significance (asterisk) is denoted as >95% C.I. from bootstrapped samples (one-sided, no adjustments). i, RFLR coefficients for mice in Fig. 5e. Bars denote mean with 95% C.I. Significance is determined using a two-sided Wilcoxon rank-sum test (*P: β = 0.05; τ = 0.014). j, Performance metrics for mice in Fig. 5f. Data are depicted as in f (*P: reward rate = 0.008; win-switch = 0.0003). k, Probability of occupancy at highly rewarded port or switching for mice in Fig. 5f. Data are depicted as in g. l, Conditional switch probabilities of the indicated treatment groups for mice in Fig. 5f. Data are depicted as in h. m, RFLR coefficients for mice in Fig. 5f. Data are depicted as in i (*P: β = 0.034; τ = 0.034).
