Fig. 3: Ach does not regulate DA dynamics during decision-making.
From: Dopamine and glutamate regulate striatal acetylcholine in decision-making
a, Schematic of TelC perturbation of Ach release. b, Epifluorescence images of TelC linked to mCherry (TelC–mCh) expressed in choline acetyltransferase (ChAT)-positive cells in the VLS (n = 12 mice). White arrowheads denote two CINs that coexpress ChAT and TelC. Scale bar, 50 µm. c, DA release as measured by carbon-fibre amperometry in an acute striatal slice containing CINs coexpressing Chrimson with mCherry (mCh) or TelC. Amperometry recordings (mean ± s.e.m.) are aligned to laser stimulation. d, Ach release in the VLS during rewarded 2ABT trials recorded with fibre photometry from mice with CINs expressing mCh or TelC in the VLS. The average ∆F/F0 of the sensor signal ± s.e.m. is shown (n = 3 mice). e, Injections and implantations (left) for fibre photometry to measure DA release (right) in the context of striatum-wide expression of mCh or TelC in CINs. Unilateral injections were performed in two separate cohorts of mice. The side entry (SE)-aligned average z-score of the sensor signal ± s.e.m. is shown (n = 5 mice per condition). f, Injections and implantations (left) for fibre photometry to measure DA release (right) in the context of VLS-selective and CIN-specific expression of TelC or mCh in separate hemispheres of the same brain (left). Data are shown as in e (n = 4 mice). g, Optogenetic inhibition of DAN cell bodies with simultaneous recordings of DA release in the VLS. Schematic of the injections and implantations (left), and summary of DA release during rewarded 2ABT trials from mice lacking opsin expression (middle) or expressing the inhibitory opsin stGtACR2 (right). The average ∆F/F0 of rDAh ± s.e.m. is indicated (n = 3 mice).
