Extended Data Fig. 2: Histology for DA and Ach recordings in the VLS with wild-type and mutant sensors.
From: Dopamine and glutamate regulate striatal acetylcholine in decision-making
a, Images of dLight1.1 expression for a representative mouse recorded for Fig. 1d and Extended Data Fig. 3d. Coronal sections show spread of expression. A higher-resolution image of the recording site (dashed box) is shown with the fibre tract denoted (inset). Scale bars (white): 1 mm; (orange): 0.2 mm. b, Location of the optical fibre tip (pink dot) for mice recorded in Fig. 1d and Extended Data Fig. 3d. c, Images of Ach3.0 expression for a representative mouse recorded for Fig. 1d and Extended Data Fig. 3d. Images depicted as in a. d, Location of the optical fibre tip (pink dot) for mice recorded in Fig. 1d and Extended Data Fig. 3d. e, Images of dLight1.1 expression for a representative mouse recorded for Extended Data Fig. 3e. Images depicted as in (a). f, Location of the optical fibre tip (pink dot) for mice recorded in Extended Data Fig. 3e. g, Images of Ach3.0 expression for a representative mouse recorded for Extended Data Fig. 3f. Images are depicted as in a. h, Schematic depicting the location of the optical fibre tip (pink dot) for mice in Extended Data Fig. 3f. i, Confocal image of dLight1.1 expression in the VLS for a representative mouse recorded for Fig. 1d and Extended Data Fig. 3d. DAPI serves as a nuclear marker. Scale bar = 10 µm. j, Confocal image of Ach3.0 expression in the VLS for a representative mouse recorded for Fig. 1d and Extended Data Fig. 3d. Images are depicted as in i. k, Images of mutant dLight1.1 expression for a representative mouse recorded for o. Images depicted as in a. l, Location of the optical fibre tip (pink dot) for mice recorded in o. m, Images of mutant Ach3.0 expression for a representative mouse recorded for p. Images are depicted as in a. n, Location of the optical fibre tip (pink dot) for mice recorded in p. o, Average ∆F/F0 of DA release during rewarded trials from dLight1.1 or its binding mutant (n = 4 mice). Mean signals ± s.e.m. are shown. p, Average ∆F/F0 of Ach release during rewarded trials from Ach3.0 or its mutant version (n = 4 mice). Signals are depicted as in o.
