Extended Data Fig. 6: Assembly of multilayer microfluidic transistor-based circuits.
From: A microfluidic transistor for automatic control of liquids

Soft-lithography techniques are used to fabricate the top and bottom PDMS layers. Top layer ports are punched into the top PDMS layer. Then, the layer is bonded with a thin silicone membrane under oxygen plasma. Ports for the bottom layer are then punched into the top layer-membrane assembly. The assembly is aligned by hand and finally bonded with the bottom layer under oxygen plasma.