Fig. 1: Model and cryo-EM images of E. amylovora DspE.
From: Bacterial pathogens deliver water- and solute-permeable channels to plant cells
a, Three-dimensional model of E. amylovora DspE generated by AlphaFold2 using MMseqs2 (ColabFold). DspE (residues 298–1838) is shown in the cartoon model in a rainbow colour gradient, with the N terminus in blue and the C terminus in red. b, Cryo-EM two-dimensional class averages of DspE, revealing a circular arrangement of domains around a pore. Scale bars, 5 nm. The result is representative of three experimental replicates. c, Surface representation of DspE. d, Sliced view of DspE. In c,d, residues are coloured on the basis of their hydrophobicity scale. The length of the proposed membrane-spanning β-barrel stem is labelled. Orange and purple asterisks mark the approximate locations of the hydrophobic cluster of L1776, L1777 and L1778 and the basic cluster of K1399 and K1401, respectively.
