Extended Data Fig. 7: Effects of PAMAM inhibitors on DspE activities in oocytes and liposome and on plant phenotypes and bacterial growth.
From: Bacterial pathogens deliver water- and solute-permeable channels to plant cells
a, Inhibition of ion currents in oocytes. DspE-dependent currents were inhibited by G0 in a dose-dependent manner across all test pulses. Solid lines represent fit values for current (µA) across the entire voltage range, with dashed lines representing the lower and upper 95% confidence interval of a quadratic polynomial regression for each treatment after subtracting control values (n = 5 oocytes). b,c, PAMAM G1 does not affect plant growth or seed production of Arabidopsis Col-0 plant. Five-week-old Col-0 plants were sprayed with 10 µM PAMAM G1 every week until 12 weeks old. b, Picture of 8-week-old plants is shown here as an example. c, Seeds were collected at 15 weeks old. No obvious difference in plant appearance or seed production was observed. Data shown as mean ± s.e.m. (n = 3 pots of plants, each pot had 4 plants). d, PAMAM G1 does not impact bacteria multiplication in vitro (in LB agar medium). Ea273 or Pst DC3000 cells in the logarithmic growth phase were spotted, at a 10-fold serial dilution (from left to right), on low-salt LB agar plates with or without 50 μM PAMAM G1. Plates were kept at 28 °C for 2 days to visualize colonies. e, Normalized liposome dye release after addition of triton. Fluorescence readings of groups with increasing concentrations of G1 were normalized to the no compound controls and are shown as mean ± s.e.m. (n = 6 batches of liposome preparations for 0.3, 1, 3, and 300 µM G1; n = 8 batches of liposome preparations for 10, 30, and 100 µM G1). Experiments were performed two (a) or three (b,c,d,e) times with similar results. Two-tailed student’s t-test (c), One-way ANOVA on Ranks (e), and regression (a) values are detailed in the Source Data files.
