Fig. 3: Inhibition of DspE and AvrE channels by the PAMAM dendrimer G1.
From: Bacterial pathogens deliver water- and solute-permeable channels to plant cells
The assays were carried out according to Fig. 2a,g, except with inhibitors added to the bath saline. a,b, DspE-dependent (a) and AvrE-dependent (b) currents were inhibited by G1. Solid lines represent fit values for current (µA) across the entire voltage range, with dashed lines representing the lower and upper 95% confidence interval of a quadratic polynomial regression for each treatment after subtracting control values. See Extended Data Fig. 4c for DspE expression in G1-containing ND96. c,d, Baseline swelling of oocytes injected with 1 ng dspE (c) or 20 ng avrE (d) cRNA was reduced in the presence of G1. Data show mean ± s.e.m. (n = 5 oocytes) increase in volume from the start point (at the time of injection) after subtracting control values. e, Inhibition of fluorescein uptake. G1 reduced fluorescein entry into oocytes expressing DspE as evaluated 6 h after injection with 1 ng dspE per oocyte. Data are presented as in Fig. 2e. f, DspE-mediated dye release from liposomes in the presence of increasing concentrations of G1. The result is representative of three experimental replicates. g, Dose-dependent inhibition of liposome dye release. IC50, half-maximal inhibitor concentration. Data show mean ± s.e.m. (n = 3 batches of liposome preparations for 0.3, 1, 3 and 300 µM G1; n = 4 batches of liposome preparations for 10, 30 and 100 µM G1). Experiments were independently carried out two times for a,b,e, four times for c,d, and seven times for f,g. P values were calculated using Two-way ANOVA (e) or two-way repeated measures ANOVA (c,d).
