Extended Data Fig. 3: Characterization of DspE-induced whole-cell currents.
From: Bacterial pathogens deliver water- and solute-permeable channels to plant cells
a,b, DspE-induced currents were not inhibited by niflumic acid or fipronil. Current values (mean ± s.e.m.; n = 5 oocytes) at different test pulses from oocytes expressing DspE proteins were recorded once in the ND96 recording buffer at 15 h after injection with 0.01 ng cRNA, and a second time after 10 min of incubation with 100 µM of each inhibitor. c, Cation replacement experiment. After 15 h of incubation, cells were recorded in the normal ND96 buffer, and then in a new recording solution where sodium in ND96 was replaced with another cation (see details in the Methods section). The data show mean ± s.e.m. (n = 5 oocytes) values at each test pulse for each cation after subtracting currents from control cells. d,e, Anion replacement experiment. Same as presented in c (mean ± s.e.m., n = 5 oocytes), but with chloride in ND96 being replaced by other elementary anions (d) the organic anion MES– (e). In e, either 100% or only 50% of the Cl– was replaced by MES–. Experiments were independently performed three (a,c,e) or two (b,d) times with similar results. Two-way ANOVA values and exact P-values for all comparisons are detailed in the Source Data files.
