Extended Data Fig. 5: DspE activities in Xenopus oocytes, Arabidopsis and liposome.
From: Bacterial pathogens deliver water- and solute-permeable channels to plant cells
a, DspE and AvrE induced a baseline swelling of many oocytes in 200 mOsm bath saline. Oocytes were imaged and measured at 24 h after 2 ng dspE or 20 ng avrE cRNA injection. Plot shows mean ± s.e.m. (n = 10 oocytes) and individual replicate values for the increased oocyte volume in relation to its initial volume. See Extended Data Fig. 4e, f for immunoblotting of DspE and AvrE proteins expressing in oocytes. b, Five-week-old Arabidopsis wild type Col-0 and transgenic Col-0/DEX::his-avrE plants (basal expression without dexamethazone induction). c, Changes in protoplast volume (mean ± s.e.m.; n = 24 protoplasts) when isolated protoplasts were incubated in protoplast incubation buffer containing 320 mM (low osmolarity) mannitol for 1 h compared to protoplast incubation buffer containing normal 400 mM mannitol for 1 h before image capture and volume analysis with Image J software. d, Liposome dye release assay using fluorescein isothiocyanate conjugated polysucrose 40 (FITC-polysucrose 40) and carboxyfluorescein (CF). e, Normalized liposome dye release (mean ± s.e.m.; n = 3 batches of liposome preparations) after addition of triton. Raw fluorescence readings were normalized to the buffer control samples. Experiments presented in this figure were independently performed two times. One-way ANOVA on Ranks (a,e) or two-tailed student’s t-test (c) values and exact P-values for all comparisons are detailed in the Source Data files.
