Extended Data Fig. 2: Portimine A triggers apoptosis and cell cycle arrest in Jurkat cells.
From: Synthesis of portimines reveals the basis of their anti-cancer activity
(a) PA induced-toxicity (24 h) can be rescued by caspase pan-inhibitor Z-VAD-FMK (50 μM) in Jurkat cells. Data are mean ± s.d. (n = 6 biologically independent samples). Statistical analysis performed using multiple unpaired two-tailed Student t-test; P-values are shown. (b-e) Cell cycle analysis in Jurkat cells. Jurkat cells were treated with 1 nM of indicated compounds for 12 h and propodium iodide used to identify different stages of cells by flow cytometry. Shown are G1 (b), S (c), G2 (d) and SubG1 (e) phase distributions. Data are mean ± s.d of three independent biologically replicated experiments. Statistical analysis was performed using one-way ANOVA analysis with multiple comparisons. P-values are shown.
