Extended Data Fig. 9: iLTD in oxytocin neurons relies on postsynaptic NMDARs and dynamin signalling.
From: Neural circuitry for maternal oxytocin release induced by infant cries
a, Whole-cell voltage-clamp recordings showing intact iLTD after repeated optogenetic stimulation of PIL terminals in PVN (‘PIL opto’) in presence of bath-applied type-III mGluR antagonist MAP4 (250 µM), for example neuron (top; p < 0.0001, Mann–Whitney two-tailed test) and summary (middle; n = 5 neurons, p = 0.02, one-sample two-tailed Student’s t-test). Bottom, IPSC/EPSC ratio in the presence of MAP4. b, iLTD in oxytocin neurons is NMDAR-dependent. Whole-cell voltage-clamp recordings showing no plasticity after PIL opto in presence of bath-applied AP5 (50 µM), for example neuron (top; p = 0.62, Mann–Whitney two-tailed test) and summary (middle; n = 8 neurons, p = 0.44, one-sample two-tailed Student’s t-test). Bottom, unchanged IPSC/EPSC ratio in the presence of AP5. c, iLTD in oxytocin neurons is dependent on postsynaptic NMDARs. Whole-cell voltage-clamp recordings showing no plasticity after PIL opto when i-MK801 (1 mM) was applied in the recording pipette, for example neuron (top; p = 0.11, Mann–Whitney two-tailed test) and summary (middle; n = 6 neurons, p = 0.18, one-sample two-tailed Student’s t-test). Bottom, unchanged IPSC/EPSC ratio in the presence of i-MK801. d, Whole-cell voltage-clamp recordings showing intact iLTD after PIL opto in presence of bath-applied OXTR antagonist OTA (1 µM), for example neuron (top; p < 0.0001, Mann–Whitney two-tailed test) and summary (middle; n = 8 neurons, p = 0.0008, one-sample two-tailed Student’s t-test). Bottom, IPSC/EPSC ratio in the presence of OTA. e,f, iLTD in oxytocin neurons is dependent on dynamin signalling. e, Whole-cell voltage-clamp recordings showing no plasticity after PIL opto when i-Dynamin inhibitor (1.5 mM) was applied in the recording pipette, for example neuron (top; p = 0.30, Mann–Whitney two-tailed test) and summary (middle; n = 7 neurons, p = 0.63, one-sample two-tailed Student’s t-test). Bottom, unchanged IPSC/EPSC ratio in the presence of i-Dynamin inhibitor. f, Whole-cell voltage-clamp recordings showing intact iLTD in oxytocin cells in the presence of a scrambled dynamin inhibitor in the recording pipette, for example neuron (top; p < 0.0001, Mann–Whitney two-tailed test) and summary (middle; n = 8 neurons, p = 0.0096, one-sample two-tailed Student’s t-test). Bottom, IPSC/EPSC ratio in the presence of a scrambled dynamin inhibitor. Data reported as mean ± s.d. *P < 0.05, **P < 0.01; ns, not significant.
