Extended Data Fig. 12: Computational lineage inference of rare ferret airway epithelial cell types using pseudotime.
From: Transgenic ferret models define pulmonary ionocyte diversity and function
a, Partition-based graph abstraction (PAGA) embedding of 1,497 rare airway epithelial cells (PNEC, tuft cells, ionocytes) and putative progenitor group (color legend), all previously identified by unsupervised clustering (Fig. 3 and Fig. 5). b, Subset of ionocytes, colored by their assignment to novel ionocyte subtypes, also by unsupervised clustering (Fig. 4). c-d, PAGA embedding of 1,497 rare airway epithelial cells, colored pseudo-time coordinate (c), and lineage transcription factors for each rare cell type (d). Trajectory (large points) and branches (color legend) in (c) were fit using elastic principal graphs.
