Extended Data Fig. 9: Migration-related ER displacement in fixed hFA and Dlxi1/2b::mScarlet+ cells.
From: Assembloid CRISPR screens reveal impact of disease genes in human neurodevelopment
a, Left: Immunofluorescence cryosection images of hFA obtained by assembly of unlabeled hCO with SEC61B-mEGFP hSO. Right: Inset showing the ER distribution in SEC61B-mEGFP+ cells migrated into the hCO. Triangles mark the nucleus and arrows mark the linear or dilated structure of the ER in the leading branch. From 2 experiments in n = 7 hFA. b, Representative time-lapse sequences showing ER displacement during the saltatory movement of a SEC61B-EGFP+ cell that was co-labelled with Dlxi1/2b::mScarlet (at ~60 days post-assembly). Triangles mark the nucleus and arrows mark the linear or dilated structure of the ER during migration (n = 5 hFA). c, Percentage of SEC61B-mEGFP and Dlxi1/2b::mScarlet double positive migratory cells showing ER displacement. Live images were taken from both hCO and hSO sides (n = 5 hFA).
